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1

Tardigrades use intrinsically disordered proteins to survive desiccation

Boothby, Thomas C., Piszkiewicz, Samantha, Holehouse, Alex, Pappu, Rohit V., Pielak, Gary J.

Journal:

Year:

2018

Language:

english

File:

PDF, 68 KB

english, 2018

2

Toxicity and Immunogenicity of a Tardigrade Cytosolic Abundant Heat Soluble Protein in Mice

Esterly, Harrison J., Crilly, Candice J., Piszkiewicz, Samantha, Shovlin, Dane J., Pielak, Gary J., Christian, Brooke E.

Journal:

Frontiers in Pharmacology

Year:

2020

File:

PDF, 527 KB

2020

3

Pressure perturbation calorimetry of helical peptides

Devin G. Barrett, C. Michael Minder, Michelle U. Mian, Shelly J. Whittington, W. John Cooper, Kristin M. Fuchs, Ashutosh Tripathy, Marcey L. Waters, Trevor P. Creamer, Gary J. Pielak

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2006

Language:

english

File:

PDF, 185 KB

english, 2006

4

Site-directed mutagenesis of cytochrome c shows that an invariant Phe is not essential for function

Pielak, Gary J., Mauk, A. Grant, Smith, Michael

Journal:

Year:

1985

File:

PDF, 333 KB

1985

5

Second virial coefficients as a measure of protein–osmolyte interactions

Gresham T. Weatherly, Gary J. Pielak

Journal:

Protein Science

Year:

2001

Language:

english

File:

PDF, 80 KB

english, 2001

6

Physicochemical Properties of Cells and Their Effects on Intrinsically Disordered Proteins (IDPs)

Theillet, Francois-Xavier, Binolfi, Andres, Frembgen-Kesner, Tamara, Hingorani, Karan, Sarkar, Mohona, Kyne, Ciara, Li, Conggang, Crowley, Peter B., Gierasch, Lila, Pielak, Gary J., Elcock, Adrian H.,

Journal:

Chemical Reviews

Year:

2014

Language:

english

File:

PDF, 2.19 MB

english, 2014

7

Quinary structure modulates protein stability in cells

Monteith, William B., Cohen, Rachel D., Smith, Austin E., Guzman-Cisneros, Emilio, Pielak, Gary J.

Journal:

Proceedings of the National Academy of Sciences

Year:

2015

Language:

english

File:

PDF, 940 KB

english, 2015

8

The structure of cytochrome c and its relation to recent studies of long-range electron transfer

Pielak, Gary J., Concar, David W., Moore, Geoffrey R., Williams, Robert J. P.

Journal:

Protein Engineering Design and Selection

Year:

1987

Language:

english

File:

PDF, 691 KB

english, 1987

9

Tardigrade Disordered Proteins Mediate Desiccation Tolerance

Boothby, Thomas C., Piszkiewicz, Samantha, Mehta, Aakash, Brozena, Alexandra, Tapia, Hugo, Koshland, Doug, Holehouse, Alex, Pappu, Rohit, Goldstein, Bob, Pielak, Gary

Journal:

Biophysical Journal

Year:

2017

Language:

english

File:

PDF, 45 KB

english, 2017

10

Magnetic Resonance Spectroscopy As a Tool for Assessing Macromolecular Structure and Function in Living Cells

Li, Conggangg, Zhao, Jiajing, Cheng, Kai, Ge, Yuwei, Wu, Qiong, Ye, Yansheng, Xu, Guohua, Zhang, Zeting, Zheng, Wenwen, Zhang, Xu, Zhou, Xin, Pielak, Gary, Liu, Maili

Journal:

Annual Review of Analytical Chemistry

Year:

2017

Language:

english

File:

PDF, 4.06 MB

english, 2017

11

Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation

Boothby, Thomas C., Tapia, Hugo, Brozena, Alexandra H., Piszkiewicz, Samantha, Smith, Austin E., Giovannini, Ilaria, Rebecchi, Lorena, Pielak, Gary J., Koshland, Doug, Goldstein, Bob

Journal:

Year:

2017

Language:

english

File:

PDF, 3.18 MB

english, 2017

12

Tardigrade Intrinsically Disordered Proteins as Potential Excipients for Biologics

Piszkiewicz, Samantha, Mehta, Aakash, Boothby, Thomas C., Daniel, William, Sheiko, Sergei, Goldstein, Bob, Pielak, Gary J.

Journal:

Biophysical Journal

Year:

2017

Language:

english

File:

PDF, 46 KB

english, 2017

13

Gelation and Vitrification of Tardigrade IDPs

Boothby, Thomas C., Piszkiewicz, Samantha, Mehta, Aakash, Brozena, Alexandra, Tapia, Hugo, Koshland, Doug, Holehouse, Alex, Pappu, Rohit, Goldstein, Bob, Pielak, Gary

Journal:

Biophysical Journal

Year:

2018

Language:

english

File:

PDF, 62 KB

english, 2018

14

Requirements for perpendicular helix pairing

Beasley, James R., Pielak, Gary J.

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

1996

Language:

english

File:

PDF, 1.33 MB

english, 1996

15

Nonideality and protein thermal denaturation

Jennifer C. Waldner, Steven J. Lahr, Marshall Hall Edgell, Gary J. Pielak

Journal:

Year:

1999

Language:

english

File:

PDF, 126 KB

english, 1999

16

Osmolyte-induced changes in protein conformational equilibria

Aleister J. Saunders, Paula R. Davis-Searles, Devon L. Allen, Gary J. Pielak, Dorothy A. Erie

Journal:

Year:

2000

Language:

english

File:

PDF, 201 KB

english, 2000

17

Probing the Micelle-Bound Aggregation-Prone State of α-Synuclein with 19F NMR Spectroscopy

Dr. Gui-Fang Wang, Dr. Conggang Li , Prof. Gary J. Pielak

Journal:

Year:

2010

Language:

english

File:

PDF, 319 KB

english, 2010

18

Internal and Global Protein Motion Assessed with a Fusion Construct and In-Cell NMR Spectroscopy

Christopher O. Barnes, William B. Monteith, Prof. Gary J. Pielak

Journal:

Year:

2011

Language:

english

File:

PDF, 194 KB

english, 2011

19

Effect of a Polyhistidine Terminal Extension on Eglin c Stability

Jennifer C. Waldner, Steven J. Lahr, Marshall Hall Edgell, Gary J. Pielak

Journal:

Analytical Biochemistry

Year:

1998

Language:

english

File:

PDF, 166 KB

english, 1998

20

Variants of saccharomyces cerevisiae iso-1 -cytochromec c at positions 18,20, 52, 82 AND 102.

Stephen F. Betz, L.Liliana Garcia, Sharon E. Hilgen, Thomas N. Sorrell, Gary J. Pielak

Journal:

Journal of Inorganic Biochemistry

Year:

1991

Language:

english

File:

PDF, 56 KB

english, 1991

21

Random mutagenesis of the N- and C-terminal helix interface of cytochrome c.

Douglas S. Auld, Zoey L. Fredericks, Gary J. Pielak

Journal:

Journal of Inorganic Biochemistry

Year:

1991

Language:

english

File:

PDF, 72 KB

english, 1991

22

Changes in global stability and local structure of cytochrome c upon substituting phenylalanine-82 with tyrosine

Rhonda M. Greene, Stephen F. Betz, Sharon Hilgen-Willis, Douglas S. Auld, Jennifer B. Fencl, Gary J. Pielak

Journal:

Journal of Inorganic Biochemistry

Year:

1993

Language:

english

File:

PDF, 950 KB

english, 1993

23

Dramatic stabilization of ferricytochrome c upon reduction

Sharon Hilgen-Willis, Gary J. Pielak, Edmond F. Bowden

Journal:

Journal of Inorganic Biochemistry

Year:

1993

Language:

english

File:

PDF, 327 KB

english, 1993

24

A bioreactor for in-cell protein NMR

Naima G. Sharaf, Christopher O. Barnes, Lisa M. Charlton, Gregory B. Young, Gary J. Pielak

Journal:

Journal of Magnetic Resonance

Year:

2010

Language:

english

File:

PDF, 499 KB

english, 2010

25

Chemical biology: Paper alert

Dale Drueckhammer, Richard Cammack, David L Pompliano, Tony Cass, Jon D Stewart, Graham R.L Cousins, Yolanda R de Miguel, Jeremy K.M Sanders, Sabine Flitsch, Philip A.S Lowden, Gary J Pielak

Journal:

Current Opinion in Chemical Biology

Year:

1999

Language:

english

File:

PDF, 78 KB

english, 1999

26

An upper limit for macromolecular crowding effects

Andrew C Miklos, Conggang Li, Courtney D Sorrell, L Andrew Lyon, Gary J Pielak

Journal:

Year:

2011

Language:

english

File:

PDF, 1.18 MB

english, 2011

27

19F NMR studies of α-synuclein-membrane interactions

Gui-Fang Wang, Conggang Li, Gary J. Pielak

Journal:

Protein Science

Year:

2010

Language:

english

File:

PDF, 307 KB

english, 2010

28

Polarity of disulfide bonds

Aleister J. Saunders, Gregory B. Young, Gary J. Pielak

Journal:

Protein Science

Year:

1993

Language:

english

File:

PDF, 158 KB

english, 1993

29

Probing weakly polar interactions in cytochrome c

Douglas S. Auld, Gregory B. Young, Aleister J. Saunders, Donald F. Doyle, Stephen F. Betz, Gary J. Pielak

Journal:

Protein Science

Year:

1993

Language:

english

File:

PDF, 1.07 MB

english, 1993

30

Amide proton exchange rates of oxidized and reduced saccharomyces cerevisiae iso-1-cytochrome c

Jennifer L. Marmorino, Douglas S. Auld, Stephen F. Betz, Donald F. Doyle, Gregory B. Young, Gary J. Pielak

Journal:

Protein Science

Year:

1993

Language:

english

File:

PDF, 979 KB

english, 1993

31

Stability of yeast iso-1-ferricytochrome c as a function of pH and temperature

David S. Cohen, Gary J. Pielak

Journal:

Protein Science

Year:

1994

Language:

english

File:

PDF, 758 KB

english, 1994

32

Design, synthesis, expression, and characterization of the genes for mouse FcγRIIb1 and FcγRIIb2 cytoplasmic regions

Lixin Chen, Nancy L. Thompson, Gary J. Pielak

Journal:

Protein Science

Year:

1997

Language:

english

File:

PDF, 2.78 MB

english, 1997

33

Baseline length and automated fitting of denaturation data

Devon L. Allen, Gary J. Pielak

Journal:

Protein Science

Year:

1998

Language:

english

File:

PDF, 166 KB

english, 1998

34

Searching for quantitative entropy-enthalpy compensation among protein variants

James R. Beasley, Donald F. Doyle, Lixin Chen, David S. Cohen, Bryan R. Fine, Gary J. Pielak

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2002

Language:

english

File:

PDF, 233 KB

english, 2002

35

In-cell protein NMR and protein leakage

Christopher O. Barnes, Gary J. Pielak

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2011

Language:

english

File:

PDF, 307 KB

english, 2011

36

Quantification of azo-coupled lysine in azo proteins by amino acid analysis

Gary J. Pielak, S. Gurusiddaiah, J.Ivan Legg

Journal:

Analytical Biochemistry

Year:

1986

Language:

english

File:

PDF, 264 KB

english, 1986

37

Reconsideration of sedimentation equilibrium distributions reflecting the effects of small inert cosolutes on the dimerization of α-chymotrypsin

Donald J. Winzor, Chetan N. Patel, Gary J. Pielak

Journal:

Biophysical Chemistry

Year:

2007

Language:

english

File:

PDF, 192 KB

english, 2007

38

Protein thermal denaturation, side-chain models, and evolution: amino acid substitutions at a conserved helix-helix interface

Pielak, Gary J., Auld, Douglas S., Beasley, James R., Betz, Stephen F., Cohen, David S., Doyle, Donald F., Finger, Shelley A., Fredericks, Zoey L., Hilgen-Willis, Sharon

Journal:

Year:

1995

Language:

english

File:

PDF, 1.08 MB

english, 1995

39

Solvent-induced collapse of α-synuclein and acid-denatured cytochrome c

Artemiza S. Morar, Alina Olteanu, Gregory B. Young, Gary J. Pielak

Journal:

Protein Science

Year:

2001

Language:

english

File:

PDF, 73 KB

english, 2001

40

Woes of proline: A cautionary kinetic tale

Journal:

Protein Science

Year:

2006

Language:

english

File:

PDF, 63 KB

english, 2006

41

Effects of molecular crowding by saccharides on α-chymotrypsin dimerization

Chetan N. Patel, Schroeder M. Noble, Gresham T. Weatherly, Ashutosh Tripathy, Donald J. Winzor, Gary J. Pielak

Journal:

Protein Science

Year:

2002

Language:

english

File:

PDF, 86 KB

english, 2002

42

Structural biology: Paper alert

Máire Convery, Caitrı́ona Dennis, Siân Rowsell, Gary J Pielak, Andreas Engel, J Bernard Heymann, Stephen Neidle, Alexis Wood, Rob Russell, Gianfranco Gilardi, Richard Newman, Sabine L Flitsch, Philip

Journal:

Current Opinion in Structural Biology

Year:

1999

Language:

english

File:

PDF, 95 KB

english, 1999

43

Proton-NMR studies show that the Thr-102 mutant of yeast iso-1-cytochrome c is a typical member of the eukaryotic cytochrome c family

Gary J. PIELAK, Jonathan BOYD, Geoffrey R. MOORE, Robert J. P. WILLIAMS

Journal:

Year:

1988

Language:

english

File:

PDF, 717 KB

english, 1988

44

Two-dimensional NMR as a probe of structural similarity applied to mutants of cytochrome c

Gary J. PIELAK, R. Andrew ATKINSON, Jonathan BOYD, Robert J. P. WILLIAMS

Journal:

Year:

1988

Language:

english

File:

PDF, 1.19 MB

english, 1988

45

The role of the internal hydrogen bond network in first-order protein electron transfer between Saccharomyces cerevisiae iso-1-cytochrome c and bovine microsomal cytochrome b5

David WHITFORD, Yuan GAO, Gary J. PIELAK, Robert J. P. WILLIAMS, George L. McLENDON, Fred SHERMAN

Journal:

Year:

1991

Language:

english

File:

PDF, 920 KB

english, 1991

46

Structural studies of yeast Iso-1 cytochrome c mutants by resonance Raman spectroscopy

Peter HILDEBRANDT, Gary J. PIELAK, Robert J. P. WILLIAMS

Journal:

Year:

1991

Language:

english

File:

PDF, 698 KB

english, 1991

47

Electron – proton coupling in cytochrome c studied using protein variants

Yuan GAO, George McLENDON, Gary J. PIELAK, Robert J. P. WILLIAMS

Journal:

Year:

1992

Language:

english

File:

PDF, 1.13 MB

english, 1992

48

Native tertiary structure in an A-state

Jennifer L. Marmorino, Melissa Lehti, Gary J. Pielak

Journal:

Journal of Molecular Biology

Year:

1998

Language:

english

File:

PDF, 303 KB

english, 1998

49

Characterization of Horse Cytochrome c Expressed in Escherichia coli

Chetan N. Patel, Maria C. Lind, Gary J. Pielak

Journal:

Protein Expression and Purification

Year:

2001

Language:

english

File:

PDF, 294 KB

english, 2001

50

Change in charge of an unvaried heme contact residue does not cause a major change of conformation in cytochrome c

Andrew G.P. Thurgood, Gary J. Pielak, Robert L. Cutler, Anne M. Davies, Colin Greenwood, A. Grant Mauk, Michael Smith, David J. Williamson, G.R. Moore

Journal:

Year:

1991

Language:

english

File:

PDF, 470 KB

english, 1991

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